Dynein Light Chain Tctex-Type 1 Modulates Orexin Signaling through Its Interaction with Orexin 1 Receptor

Dynein Light Chain Tctex-Type 1 Modulates Orexin Signaling through Its Interaction with Orexin 1 Receptor

Orexins (OX-A, OX-B) are neuropeptides involved in the regulation of the sleep-wake cycle, feeding and reward, via activation of orexin receptors 1 and 2 (OX1R, OX2R). The loss of orexin peptides or functional OX2R has been shown to cause the sleep disorder, narcolepsy. Since the regulation of orexin receptors remains largely undefined, we searched for novel protein partners of the intracellula...

G protein bc subunit interaction with the dynein light-chain component Tctex-1 regulates neurite outgrowth

Pallavi Sachdev, Santosh Menon, David B Kastner, Jen-Zen Chuang, Ting-Yu Yeh, Cecilia Conde, Alfredo Caceres, Ching-Hwa Sung and Thomas P Sakmar* Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, New York, NY, USA, Department of Ophthalmology, Weill Medical College of Cornell University, New York, NY, USA, INIMEC-CONICET, Cordoba, Argentina and Department of Cell and...

Interaction of p59 Kinase with the Dynein Light Chain, Tctex-1, and Colocalization During Cytokinesis

Interaction of p59fyn kinase with the dynein light chain, Tctex-1, and colocalization during cytokinesis.

The protein tyrosine kinase p59fyn (Fyn) plays important roles in both lymphocyte Ag receptor signaling and cytokinesis of proB cells. We utilized yeast two-hybrid cloning to identify the product of the tctex-1 gene as a protein that specifically interacts with Fyn, but not with other Src family kinases. Tctex-1 was recently identified as a component of the dynein cytoskeletal motor complex. Th...

Orexin-A regulates cell apoptosis in human H295R adrenocortical cells via orexin receptor type 1 through the AKT signaling pathway.

Numerous studies have demonstrated the ability of orexin-A to regulate adrenocortical cells through the mitogen-activated protein kinase signaling pathway. In the present study, human H295R adrenocortical cells were exposed to orexin‑A (10‑10-10‑6 M), with orexin receptor type 1 (OX1 receptor) antagonist SB334867 or AKT antagonist PF‑04691502. It was found that orexin‑A stimulated H295R cell pr...